DNA Extractor (R) SP kit (血清、血漿的DNA提取試劑盒)Grade: for Genetic Research
Storage condition: Keep at 2-10 degrees C.
Manufacturer: Wako Pure Chemical Industries, Ltd. (用于存在于血清或血漿中DNA片斷的提取)Cat. No. Pkg. Size
296-60501 50Tests Summary
This product is for research use only. Do not administer it to human.DNA extraction from serum and plasmaDNA Extractor SP Kit efficiently extracts DNA fragments contained in serum and plasma. Based on the Sodium Iodide (NaI) method1, this kit enables the whole procedures done in a single micro-centrifuge tube without using hazardous phenol/chloroform.
Recently, tumor-specific genes have been amplified and detected in the serum and plasma of patients with various diseases such as lung, breast and colon cancer. Many of these reference articles are currently being published2,3,4.
The kit is a powerful pretreatment reagent for the detection and analysis of target DNA because of its high quality and yield.
Features
High DNA yield (about 100%) from small amount of serum and plasma
The DNA extraction procedure is based on a simple centrifugal separation using Sodium Iodide and alcohol, that results in less loss of DNA.Safe operation
No phenol or chloroform required.Minimum contamination: The entire extraction of DNA can be done in a single centrifuge tubeComplete removal of lipid derived from bloodLess variability in extraction from sample to sampleKit Contents (50 reactions)Enzyme reaction solution 1 x 10 mLProtein digestion solution 1 x 250 uL (uL: micro-L)Sodium iodide solution 1 x 15 mLAlcohol solution 1 x 30 mLWashing Solution (A) 1 x 50 mLWashing Solution (B) 1 x 50 mLReferencesIshizawa, M., Kobayashi, Y., Miyamura, T. and Matsuura, S.: Nucleic Acids Res., 22, 1774 (1994).Sozzi, G., Musso, K., Ratcliffe, C., Goldstraw, P., Pierotti, M. A. and Pastorino, U.: Clin. Cancer Res., 5, 2689 (1999)Silva, J. M., Dominguez, G., Garcia, J. M., Gonzalez, R., Villanueva M. J., Navarro, F., Provencio, M., San, Martin, S., Espana, P. and Bonilla, F.: Cancer Res., 59, 3251 (1999)Shao, Z. M., Wu, J., Shen Z. Z. and Nguyen, M.: Clin. Cancer Res., 7, 2222 (2001) 如有更多其它試劑產(chǎn)品需求與查詢,請(qǐng)及時(shí)接洽:info@express-cn.com 或在我公司查詢主頁客戶留言處(http://www.express-cn.com/expressgb/lyb.htm)留下您的查詢內(nèi)容,我們即會(huì)在當(dāng)天為您作出回復(fù)。伊普瑞斯科技有限公司
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DNA Extractor (R) SP kit (血清、血漿的DNA提取試劑盒)