TOSOH TSKgel Amide-80正相與親水作用色譜柱
反相是應(yīng)用zui廣的HPLC保留方式,但極性很大的化合物常常不能充分保留在低百分比有機(jī)相中,甚至不能保留在100%含水流動相中。對于含有相同組分的樣品,正相洗脫順序與RPC中的洗脫順序正好相反。盡管正相LC在傳統(tǒng)上采用非極性有機(jī)流動相和硅固定相,但如今,多數(shù)正相分離法均采用含水有機(jī)流動相和極性較強的鍵合固定相。該HPLC方式目前普遍稱為HILIC,即親水作用色譜法。
如果采用強極性鍵合相(如TSKgel Amide-80),正相或親水作用色譜法可改善極性化合物的保留行為,其保留機(jī)理是使用乙腈與乙酸銨緩沖液的混合流動相。與反相保留行為相反,在HILIC中,通過增加乙腈百分比可更長時間地保留樣品中的極性成分。
訂貨信息
TSKgel Amide-80 3µm分析柱
貨號 |
描述 |
粒徑 |
材質(zhì) |
內(nèi)徑 (mm) |
長度(cm) |
021864 |
TSKgel Amide-80 3µm |
3 µm |
Stainless Steel |
2 |
5 |
021865 |
TSKgel Amide-80 3µm |
3 µm |
Stainless Steel |
2 |
15 |
022850 |
TSKgel Amide-80 3µm |
3 µm |
Stainless Steel |
3 |
5 |
022851 |
TSKgel Amide-80 3µm |
3 µm |
Stainless Steel |
3 |
10 |
022852 |
TSKgel Amide-80 3µm |
3 µm |
Stainless Steel |
3 |
15 |
021862 |
Guard Cartridge for 2 and 3mmID columns, 3pk |
3 µm |
Stainless Steel |
2 |
1 |
019308 |
Guard Cartridge Holder for P/N 0021862 |
|
Stainless Steel |
2 |
1 |
021866 |
TSKgel Amide-80 3µm |
3 µm |
Stainless Steel |
4.6 |
5 |
022849 |
TSKgel Amide-80 3µm |
3 µm |
Stainless Steel |
4.6 |
10 |
021867 |
TSKgel Amide-80 3µm |
3 µm |
Stainless Steel |
4.6 |
15 |
021863 |
Guard Cartridge for 4.6mmID columns, 3pk |
3 µm |
Stainless Steel |
3.2 |
1.5 |
019018 |
Guard Cartridge Holder for P/N 0021863 |
|
Stainless Steel |
3.2 |
1.5 |
TSKgel Amide-805µm分析柱
貨號 |
描述 |
粒徑 |
材質(zhì) |
內(nèi)徑 (mm) |
長度 (cm) |
020009 |
TSKgel Amide-80 |
5 µm |
Stainless Steel |
1 |
5 |
020010 |
TSKgel Amide-80 |
5 µm |
Stainless Steel |
1 |
10 |
021486 |
TSKgel Amide-80 |
5 µm |
Stainless Steel |
1 |
15 |
021487 |
TSKgel Amide-80 |
5 µm |
Stainless Steel |
1 |
25 |
019694 |
TSKgel Amide-80 |
5 µm |
Stainless Steel |
2 |
5 |
019695 |
TSKgel Amide-80 |
5 µm |
Stainless Steel |
2 |
10 |
019696 |
TSKgel Amide-80 |
5 µm |
Stainless Steel |
2 |
15 |
019697 |
TSKgel Amide-80 |
5 µm |
Stainless Steel |
2 |
25 |
0021941 |
Guard Cartridge for 2mm ID columns, 3 pk, Replaces P/N 0042140 |
5 µm |
Stainless Steel |
2 |
1 |
019308 |
Guard Cartridge holder for P/N 0021941 |
|
Stainless Steel |
2 |
1 |
019532 |
TSKgel Amide-80 |
5 µm |
Stainless Steel |
4.6 |
5 |
019533 |
TSKgel Amide-80 |
5 µm |
Stainless Steel |
4.6 |
10 |
013071 |
TSKgel Amide-80 |
5 µm |
Stainless Steel |
4.6 |
25 |
019021 |
Guard Column for 4.6mm ID columns |
5 µm |
Stainless Steel |
4.6 |
1 |
019010 |
Guard Column for 4.6mm ID columns, 3 pk |
5 µm |
Stainless Steel |
3.2 |
1.5 |
0019018 |
Guard Cartridge holder for P/N 0019010 |
|
Stainless Steel |
3.2 |
1.5 |
014459 |
TSKgel Amide-80 |
10 µm |
Stainless Steel |
7.8 |
30 |
014460 |
TSKgel Amide-80 |
10 µm |
Stainless Steel |
21.5 |
30 |
014461 |
Guard column for P/N 0014460 |
10 µm |
Stainless Steel |
21.5 |
7.5 |