TOYOPEARL AF-Blue HC650M 親和填料是一種非常有效的蛋白質(zhì)純化方法。其分離的機(jī)制是基于蛋白質(zhì)和某些特定分子具有特異性的相互作用。除了生物特異性配體外（如酶底物、抗體、受體等等），一些俗稱為類生物配體（如凝集素、染料）的物質(zhì)也用在親和層析中，而在固定化的金屬離子上進(jìn)行的類親和層析在蛋白質(zhì)和多肽的分離純化工作中越來越重要。 親和層析的選擇性和效率極高。
一般來說, TOYOPEARL AF-Tresyl-650M和TOYOPEARL AF-Formyl-650M被推薦用來偶聯(lián)蛋白質(zhì), 而TOYOPEARL AF-Epoxy-650M適合連結(jié)小分子配體, TOYOPEARL AF-Amino-650M和TOYOPEARL AF-Carboxyl-650M既可以偶聯(lián)蛋白質(zhì)也可以用來連接小分子配體。
TOYOPEARL親和樹脂所能偶聯(lián)的代表性物質(zhì)的偶聯(lián)密度
結(jié)合了特異性配體的TOYOPEARL親和樹脂
TOYOPEARL AF-Chelate-650M樹脂
對(duì)于TOYOPEARL AF-Chelate-650M來說，共價(jià)連接在介質(zhì)上的亞氨基二乙酸（IDA）是金屬離子的配體，可以結(jié)合各種金屬離子(常用的為Ca2+, Ni2+, Zn2+, Co2+)，而金屬離子的空軌道可以用來螯合特定的含有組氨酸和半胱氨酸的蛋白質(zhì)和多肽。常用于純化人重組生長因子, 組織蛋白酶原促進(jìn)因子,血型糖蛋白和整體細(xì)胞.
TOYOPEARL AF Blue HC-650M樹脂
功能團(tuán)為Cibachron Blue F3GA. 結(jié)合蛋白容量高,尤其適合純化白蛋白.
TOYOPEARL AF-Red 650M樹脂
功能團(tuán)為Procion Red HE-3B,適合純化核苷依賴性酶, 血漿酶原脂蛋白,多肽, 荷爾蒙和細(xì)胞毒素
部分產(chǎn)品型號(hào)如下：
|
貨號(hào) |
生產(chǎn)商 |
描述 |
|
008000 |
TOSOH |
AF-Epoxy-650M 10G |
|
008002 |
TOSOH |
AF-Amino-650M 25mL |
|
008004 |
TOSOH |
AF-Formyl-650M 25mL |
|
008006 |
TOSOH |
AF-Carboxy-650M 25mL |
|
008038 |
TOSOH |
AF-Epoxy-650M 100G |
|
008039 |
TOSOH |
AF-Amino-650M 100mL |
|
008040 |
TOSOH |
AF-Formyl-650M 100mL |
|
008041 |
TOSOH |
AF-Carboxy-650M 100mL |
|
008651 |
TOSOH |
AF-Red-650ML 25mL |
|
014471 |
TOSOH |
AF-Tresyl-650M 5G |
|
014472 |
TOSOH |
AF-Tresyl-650M 100G |
|
014475 |
TOSOH |
AF-Chelate-650M 25mL |
|
014905 |
TOSOH |
AF-Tresyl-650M 200G |
|
014906 |
TOSOH |
AF-Tresyl-650M 1000G |
|
014907 |
TOSOH |
AF-Chelate-650M 1L |
|
014908 |
TOSOH |
AF-Chelate-650M 5L |
|
017396 |
TOSOH |
AF-Formyl-650M 1L |
|
017397 |
TOSOH |
AF-Formyl-650M 5L |
|
018074 |
TOSOH |
AF-Amino-650M 1L |
|
018315 |
TOSOH |
AF-Epoxy-650M 1000G |
|
018316 |
TOSOH |
AF-Amino-650M 5L |
|
018827 |
TOSOH |
AF-Carboxy-650M 1L |
|
018828 |
TOSOH |
AF-Carboxy-650M 5L |
|
019688 |
TOSOH |
AF-Blue HC650M 25mL |
|
019689 |
TOSOH |
AF-Blue HC650M 100mL |
|
019690 |
TOSOH |
AF-Blue HC650M 1L |
|
019691 |
TOSOH |
AF-Blue HC650M 5L |
|
019800 |
TOSOH |
AF-Chelate-650M 100mL |
|
019801 |
TOSOH |
AF-Red-650ML 100mL |
|
020030 |
TOSOH |
AF-Heparin HC-650M 10mL |
|
020031 |
TOSOH |
AF-Heparin HC-650M 100mL |
|
020032 |
TOSOH |
AF-Heparin HC-650M 1L |
|
020033 |
TOSOH |
AF-Heparin HC-650M 5L |
|
021384 |
TOSOH |
ToyoScreen AF-Chelate-650M, 1mL*6 |
|
021385 |
TOSOH |
ToyoScreen AF-Chelate-650M, 5mL*6 |
|
021386 |
TOSOH |
ToyoScreen AF-Blue HC-650M, 1mL*6 |
|
021387 |
TOSOH |
ToyoScreen AF-Blue HC-650M, 5mL*6 |
|
021388 |
TOSOH |
ToyoScreen AF-Red-650M, 1mL*6 |
|
021389 |
TOSOH |
ToyoScreen AF-Red-650M, 5mL*6 |
|
021390 |
TOSOH |
ToyoScreen AF-Heparin HC-650M, 1mL*6 |
|
021391 |
TOSOH |
ToyoScreen AF-Heparin HC-650M, 5mL*6 |
|
042102 |
TOSOH |
AF-Red-650ML 1L |
|
043400 |
TOSOH |
AFFIPAK ACT (AF-Epoxy, Tresyl-650M) , 65μm |
|
043402 |
TOSOH |
AF-Epoxy-650M 5G |
|
043410 |
TOSOH |
AFFIPAK (AF-Amino, Carboxyl, Formyl-650 M), 65μm |
|
043411 |
TOSOH |
AF-Amino-650M 10mL |
|
043412 |
TOSOH |
AF-Carboxy-650M 10mL |
|
043413 |
TOSOH |
AF-Formyl-650M 10mL |