6.8.1 HPLC chromatographic system/condition 高效液相色譜系統(tǒng)/條件
Chromatographic column: A stainless steel column packed with a irregular, porous silica gel to which benzene sulphonic acid groups have been chemically bonded (10 μm) or equivalent.
色譜柱:填充不規(guī)則多孔硅膠,化學(xué)鍵合苯磺酸基團(tuán)的不銹鋼柱(10 μm)或類似柱。
Size 尺寸:4.6 mm × 25 cm
Wavelength 波長:232 nm
Injection volume 進(jìn)樣量:20 μL
Flow rate 流速:1 mL/min
Mobile Phase: 1.7% w/v solution of ammonium dihydrogen orthophosphate adjusted to pH 3.0 with orthophosphoric acid.
流動(dòng)相:1.7%w/v磷酸二氫銨溶液,用磷酸調(diào)節(jié)pH至3.0。
供應(yīng) 鹽酸二甲雙胍分析用色譜柱