超高速視頻級(jí)原子力顯微鏡（Sample-Scanning High-Speed Atomic Force Microscope ，HS-AFM SS-NEX）是由日本 Kanazawa 大學(xué) Prof. Ando 教授團(tuán)隊(duì)歷經(jīng)數(shù)十年研發(fā)而成的，也是世界上第一臺(tái)可以達(dá)到視頻級(jí)成像的商業(yè)化原子力顯微鏡。
3. High-speed atomic force microscopy shows that annexin V stabilizes membranes on the second timescale
4. IgGs are made for walking on bacterial and viral surfaces
5. Long-tip high-speed atomic force microscopy for nanometer-scale imaging in live cells
6. High-speed atomic force microscopy shows dynamic molecular processes in photoactivated bacteriorhodopsin
7. Tuning crystallization pathways through sequence engineering of biomimetic polymers
8. Lipid-bilayer-assisted two-dimensional self-assembly of DNA origami nanostructures
相較于目前市場(chǎng)上的原子力顯微鏡成像設(shè)備，HS-AFM突破了 “掃描成像速慢”的限制，掃描速度最高可達(dá) 20 frame/s，并且有 4 種掃描臺(tái)可供選擇。樣品無(wú)需特殊固定染色，不影響生物分子的活性，尤其適用于生物大分子互作動(dòng)態(tài)觀測(cè)。液體環(huán)境下直接檢測(cè)，超快速動(dòng)態(tài)成像，分辨率為納米水平。探針小，適用于生物樣品；懸臂探針共振頻率高，彈簧系數(shù)小，避免了對(duì)生物樣品等的損傷。懸臂探針可自動(dòng)漂移校準(zhǔn)，適用于長(zhǎng)時(shí)間觀測(cè)。采用動(dòng)態(tài)PID控制，高速掃描時(shí)仍可獲得清晰的圖像。XY軸分辨率2nm；Z軸分辨率0.5nm。
超高速視頻級(jí)原子力顯微鏡HS-AFM推出至今，全球已有80多位用戶，發(fā)表 SCI 文章 200 余篇，包括Science, Nature, Cell 等頂級(jí)雜志。
HS-AFM超高速視頻級(jí)原子力顯微鏡應(yīng)用案例：
1.Video imaging of walking myosin V 實(shí)時(shí)觀察myosin V蛋白的運(yùn)動(dòng)
N. Kodera et al. Nature 468, 72 (2010). Kanazawa University
2.Real-space and real-time dynamics of CRISPR-Cas9 實(shí)時(shí)顯示CRISPR基因編輯
Mikihiro et al. Nature Communications, (2017). Kanazawa University
3. High-speed atomic force microscopy shows that annexin V stabilizes membranes on the second timescale
Miyagi A, et al. Nature Nanotechnology (2016)
4. IgGs are made for walking on bacterial and viral surfaces
J Preiner, et al. Nature Communications(2014)
5. Long-tip high-speed atomic force microscopy for nanometer-scale imaging in live cells
Mikihiro Shibata, et al. Scientific Reports(2015)
6. High-speed atomic force microscopy shows dynamic molecular processes in photoactivated bacteriorhodopsin
Shibata M, et al. Nature Nanotechnology (2010)
7. Tuning crystallization pathways through sequence engineering of biomimetic polymers
Xiang Ma, et al. Nature Materials (2017)
8. Lipid-bilayer-assisted two-dimensional self-assembly of DNA origami nanostructures
Yuki Suzuki, et al. Nature Communications(2015)
HS-AFM超高速視頻級(jí)原子力顯微鏡設(shè)備規(guī)格及配置參數(shù)：
基本參數(shù)：
SS-NEX 型可選配置：
