DNA Extractor (R) SP kit (血清、血漿的DNA提取試劑盒)
Grade: for Genetic Research
Storage condition: Keep at 2-10 degrees C.
Manufacturer: Wako Pure Chemical Industries, Ltd.
(用于存在于血清或血漿中DNA片斷的提取)
Cat. No. Pkg. Size
296-60501 50Tests
Summary
This product is for research use only. Do not administer it to human.
DNA extraction from serum and plasma
DNA Extractor SP Kit efficiently extracts DNA fragments contained in serum and plasma. Based on the Sodium Iodide (NaI) method1, this kit enables the whole procedures done in a single micro-centrifuge tube without using hazardous phenol/chloroform.
Recently, tumor-specific genes have been amplified and detected in the serum and plasma of patients with various diseases such as lung, breast and colon cancer. Many of these reference articles are currently being published2,3,4.
The kit is a powerful pretreatment reagent for the detection and analysis of target DNA because of its high quality and yield.
Features
High DNA yield (about 100%) from small amount of serum and plasma
The DNA extraction procedure is based on a simple centrifugal separation using Sodium Iodide and alcohol, that results in less loss of DNA.
Safe operation
No phenol or chloroform required.
Minimum contamination: The entire extraction of DNA can be done in a single centrifuge tube
Complete removal of lipid derived from blood
Less variability in extraction from sample to sample
Kit Contents (50 reactions)
Enzyme reaction solution 1 x 10 mL
Protein digestion solution 1 x 250 uL (uL: micro-L)
Sodium iodide solution 1 x 15 mL
Alcohol solution 1 x 30 mL
Washing Solution (A) 1 x 50 mL
Washing Solution (B) 1 x 50 mL
References
Ishizawa, M., Kobayashi, Y., Miyamura, T. and Matsuura, S.: Nucleic Acids Res., 22, 1774 (1994).
Sozzi, G., Musso, K., Ratcliffe, C., Goldstraw, P., Pierotti, M. A. and Pastorino, U.: Clin. Cancer Res., 5, 2689 (1999)
Silva, J. M., Dominguez, G., Garcia, J. M., Gonzalez, R., Villanueva M. J., Navarro, F., Provencio, M., San, Martin, S., Espana, P. and Bonilla, F.: Cancer Res., 59, 3251 (1999)
Shao, Z. M., Wu, J., Shen Z. Z. and Nguyen, M.: Clin. Cancer Res., 7, 2222 (2001)
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