酪胺酸酶Tyrosinase from mushroom
BioChemika, lyophilized, ≥2000 units/mg
Descriptions
Other Notes Kinetic study of the suicide inactivation of tyrosinase1 ; Chromogenic assay
Unit Definition 1 U corresponds to the amount of enzyme which increases the absorbance at 305 nm by 0.001 per minute at pH 6.5 and 25°C (L-tyrosine as substrate, 3.0 mL reaction mix); 30 absorbance-U as described above are equivalent to ~1 U [when 1 U is the amount of enzyme which oxidyzes 1 μmol 4-methylcatechol per minute at pH 6.5 and 25°C]
General description Isoelectric point (pI): 4.7-5
pH optimum is 6-7
Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis.
Tyrosinase is a copper-containing oxidase, which has activity for both catechols and cresol. It is responsible for browning reactions. The enzyme is reported to have two binding sites for aromatic substrates and a different binding site for oxygen-copper. The copper is probably Cu(I), with inactivation involving oxidation to Cu(II) ion.
Properties
mol wt
Mr ~125000
solubility
0.02 M phosphate, pH 6.5: 5mg/mL, clear, slightly brown to deep brown
storage temp.
?20°C
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
如有更多其它試劑產(chǎn)品需求與查詢,請及時(shí)接洽:info@express-cn.com 或在我公司查詢主頁客戶留言處(http://www.express-cn.com/expressgb/lyb.htm)留下您的查詢內(nèi)容,我們即會在當(dāng)天為您作出回復(fù)。
伊普瑞斯科技有限公司
info@express-cn.com
在線客服:QQ:1069267096
MSN:liyuqiu1983@hotmail.com
cindylee-1981@hotmail.com
總機(jī):010-60206266
傳真:010-60206773
www.express-cn.com